Cloned (Comment) | Organism |
---|---|
- |
Volvariella volvacea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 0.1 mM, 100% inhibition | Volvariella volvacea | |
cysteine | 0.1 mM, 100% inhibition | Volvariella volvacea | |
dithiothreitol | 0.1 mM, 100% inhibition | Volvariella volvacea | |
EDTA | 1 mM, 20% inhibition | Volvariella volvacea | |
thioglycolic acid | 1 mM, 100% inhibition | Volvariella volvacea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
syringaldazine | pH 5.6, 45°C | Volvariella volvacea | |
0.03 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.0, 45°C | Volvariella volvacea | |
0.57 | - |
2,6-dimethoxyphenol | pH 4.6, 45°C | Volvariella volvacea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Volvariella volvacea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Volvariella volvacea | - |
growth medium supplemented with copper | - |
Purification (Comment) | Organism |
---|---|
- |
Volvariella volvacea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Volvariella volvacea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
substrate 2,6-dimethoxyphenol, pH 4.6 | Volvariella volvacea |
3.4 | - |
substrate syringaldazine, pH 5.6 | Volvariella volvacea |
13.5 | - |
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.0 | Volvariella volvacea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Volvariella volvacea | ? + H2O | - |
? | |
2,6-dimethoxyphenol + H2O | about 5% of the activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Volvariella volvacea | ? | - |
? | |
additional information | poor substrates: guaiacol, catechol, no substrate: tyrosine, L-3,4-dimethoxyphenol, dihydroxyphenylalanine | Volvariella volvacea | ? | - |
? | |
syringaldazine + O2 | about 17% of the activity with 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Volvariella volvacea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Volvariella volvacea |
More | N-terminal amino acid sequence | Volvariella volvacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Volvariella volvacea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Volvariella volvacea |
4.6 | - |
substrate 2,6-dimethoxyphenol | Volvariella volvacea |
5.6 | - |
substrate syringaldazine | Volvariella volvacea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Volvariella volvacea | isoelectric focusing | - |
4.2 |